PH Buffered NAD+ 5,000MG Kit

About PH Buffered NAD+ 5,000MG Kit

Nicotinamide adenine dinucleotide (NAD+) is a coenzyme present in every living cell, where it functions as the primary electron carrier in redox reactions. It is not a peptide — it is a dinucleotide, built from nicotinamide and adenine riboside units joined by phosphate groups.

Its research prominence comes from two roles: as the redox cofactor driving cellular energy metabolism, and as the required substrate for sirtuins and PARP enzymes, which consume it during DNA repair and gene regulation. Cellular NAD+ declines with age, which is the basis of most longevity research interest.

Supplied for laboratory research use only. Not for human consumption.

Research background

Redox cofactor function

NAD+ cycles between oxidised (NAD+) and reduced (NADH) states, carrying electrons through glycolysis, the citric acid cycle and oxidative phosphorylation. The ratio between the two forms is itself a regulated signal.

Sirtuin substrate

Sirtuins are NAD+-dependent deacetylases involved in gene regulation and metabolic adaptation. Because they consume NAD+ rather than merely binding it, their activity is directly limited by NAD+ availability — the central observation behind NAD+ research.

DNA repair and PARP activity

PARP enzymes also consume NAD+ during DNA damage response. Extensive DNA damage depletes the cellular pool, creating competition between repair and sirtuin activity that researchers have examined in detail.

Precursor comparisons

A substantial literature compares direct NAD+ with precursors such as nicotinamide riboside and nicotinamide mononucleotide, examining which route most effectively raises intracellular levels.

Reconstitution

1. Equilibrate firstAllow the vial to reach room temperature before opening. Introducing solvent into a cold vial promotes condensation, which affects both concentration accuracy and stability.
2. Select the diluentBacteriostatic water — sterile water with 0.9% benzyl alcohol — is standard where the solution will be held for more than a few days. Plain sterile water is appropriate only for single-session use with no storage.
3. Add slowly, down the wallDirect the solvent against the inner wall of the vial rather than onto the powder. A stream striking the lyophilized cake causes localized shear that can denature peptide chains.
4. Do not shakeSwirl gently, or simply let the vial stand. Agitation introduces air and mechanical stress, and both degrade peptides. Full dissolution usually takes a minute or two.
5. Inspect before useA correctly reconstituted solution is clear and colourless. Cloudiness, visible particulate or discolouration indicates a problem with the material or the diluent.

Storage and stability

Lyophilized peptide is stable enough to survive shipping at ambient temperature, which is why cold-chain courier service is not required for transit. Long-term storage is a different matter — material intended to be held for months belongs in a freezer.

Protect from light, and avoid repeated freeze-thaw cycles. Each cycle causes measurable degradation, so dividing a reconstituted stock into single-use aliquots before freezing is preferable to thawing and refreezing one vial repeatedly.

Purity and verification

Every lot is tested by an independent laboratory using two orthogonal methods. HPLC (high-performance liquid chromatography) establishes purity as a percentage of total peptide content, separating the target compound from truncated sequences and synthesis by-products. LC-MS (liquid chromatography–mass spectrometry) confirms molecular identity by mass.

Both matter. Purity alone is insufficient — a 99% pure sample of the wrong compound is still the wrong compound. Together the two tests establish identity and quality, which is why NAD+ is released only after both pass.

The certificate of analysis references the lot number printed on your vial label. Request the COA for your lot at [email protected].

Published research

Peer-reviewed studies indexed in PubMed. Each links to the abstract and, where a free copy exists, to the full text. We link primary sources rather than summarising them second-hand.

Frequently asked questions

Related compounds

Compounds studied alongside this one, or acting through related pathways.

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